Male Infertility and Sperm DNA Fragmentation

Clinical aspects of sperm DNA fragmentation detection and male infertility.

Over the past 25 years, various methods have been developed to measure sperm DNA strand breaks in situ. Currently, there are four major tests of sperm DNA fragmentation, including the Comet, Tunel, sperm chromatin structure assay (SCSA) and the acridine orange test (AOT). The Comet assay is a light microscope technique where the sperm cells are mixed with melted agarose and then placed on a gla...

Implications of ejaculatory sperm DNA fragmentation on male infertility management

Should sperm DNA fragmentation testing be included in the male infertility work-up?

A response to the editorial 'Are we ready to incorporate sperm DNA fragmentation testing into our male infertility work-up? A plea for more robust studies' by Erma Drobnis and Martin Johnson.

Sperm DNA fragmentation testing in male infertility work-up: are we ready?

Is testing of sperm DNA fragmentation (SDF) ready for the basic work-up of male infertility?

The authors have presented an informative manuscript on testing of sperm DNA fragmentation (SDF). They presented background material on the development of these tests, and their discussions were supported by 129 references from PubMed up to June 2016. In the section entitled “Evidence Synthesis”, they reported on 8 SDF tests that have been used clinically. The final section was called “Indicati...